Relationships between batch study results and protein properties were explored based on single property and aggregated property information to move towards determining which sets of protein property information would be predictive of protein behavior in hydrophobic chromatography. We have not yet reached our goal of being able to predict structural change on interaction with RP C18 media based on specific protein properties. This would require the collection of additional batch adsorption data from more proteins and with varied adsorbed concentration. However, we have gained valuable insight into which protein characteristics and properties are useful towards this goal and the interconnectedness of RP behavior.A second issue with the existing HEAPS S reference spectra involves the parsing of the amide I band intensities into ... Raman spectra for unordered helix and sheet could not be distinguished from each other using structure fractions asanbsp;...
|Title||:||Understanding Protein Structural Change in Hydrophobic Chromatography|
|Author||:||Abigail H. Laurent|
|Publisher||:||ProQuest - 2008|