Established leaders in trinucleotide repeat disease describe in step-by-step detail their best techniques for studying trinucleotide pathology at the molecular level. The protocols cover a variety of targets, ranging from DNA and RNA to proteins and whole animals, and focus not only on causal genes, but also on their consequent affected products, such as transcription factors, neurotransmitter receptors, proteasomes, and mitochondria/oxidation damage.manual for quantification of transcripts from other repeat-containing genes or gene transcript quantification in general. The TaqMan system is ... Microfast track mRNA isolation kit (Invitrogen). 5. ... TOPO TA-cloning kit (Invitrogen). 8. Plasmid anbsp;...
|Title||:||Trinucleotide Repeat Protocols|
|Publisher||:||Springer Science & Business Media - 2004-01-01|