Understanding how the brain functions is one of the most ambitious current scientific goals. This challenge will only be accomplish by a multidisciplinary approach involving genetics, molecular biology, optics, ethology, neurobiology and mathematics and using tractable model systems. The zebrafish larva is a transparent genetically tractable small vertebrate, ideal for the combination state-of-the- art imaging techniques (e.g. two-photon scanning microscopy, single-plane illumination microscopy, spatial light modulator microscopy and lightfield microscopy), bioluminiscence and optogenetics to monitor and manipulate neuronal activity from single specific neurons up to the entire brain, in an intact behaving organism. Furthermore, the zebrafish model offers large and increasing collection of mutant and transgenic lines modelling human brain diseases. With these advantages in hand, the zebrafish larva became in the recent years, a novel animal model to study neuronal circuits and behaviour, taking us closer than ever before to understand how the brain controls behaviour.Each stack was analyzed independently using the same algorithm as in single z- plane recordings. AUTOMATIC IMAGE SEGMENTATION A segmentation routine written in Matlab was developed in order to automatically identify the regions of interest (ROIs) corresponding to individual somata and neuropil regions (the Matlab code is provided as a Supplementary ... A watershed algorithm was implemented on the resulting smoothed gray-scale image, returning a collection of adjacentanbsp;...
|Title||:||The world according to zebrafish: How neural circuits generate behaviour|
|Author||:||Gonzalo G. De Polavieja, German Sumbre|
|Publisher||:||Frontiers E-books - 2014-11-07|