Ligation of the vector with a PCR product containing 3a#39; overhangs is very efficient and occurs spontaneously within 5 minutes at room temperature (TOPO TA Cloning Version B Manual, Invitrogen, p. I). The TOPO-Cloning Reaction can then beanbsp;...
|Title||:||Systems and technologies for clinical diagnostics and drug discovery II|
|Author||:||Gerald E. Cohn, John C. Owicki|
|Publisher||:||SPIE-International Society for Optical Engineering - 1999|