In addition to being the natural genetic information carrier, DNA can also serve as a versatile material for construction of nanoscale objects. By using the base-pairing properties of DNA, we have been able to mass-produce nano-scale structures in a variety of different shapes, upon which patterns of other molecules can be further specified. The diversity of molecules and materials that can be attached to DNA and the capability of providing precise spatial positioning considerably enhance the attractiveness of DNA for nano-scale construction. A further challenge remains to use these DNA based structures for biomedical applications.1 I¼l of salt solution (provided in Topo cloning kit; stored at a20AdC) 1 I¼l of dH20 1 I¼l of TOPO vector (also stored at ... cells, using the conventional ice-heatshock method detailed in the manual (#C4040-03, Invitrogen, Carlsbad, California).
|Title||:||Self-assembled DNA Nanostructures: From Structural Material to Biomedical Nanodevices|
|Publisher||:||ProQuest - 2008|