Ruthenium-mediated DNA Oxidation in Condensed Media

Ruthenium-mediated DNA Oxidation in Condensed Media

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DNA oxidation is a process that has been implicated in mutagenesis and cancer. Previous studies to determine the pathways and products of DNA oxidation have been carried out in dilute aqueous solutions or organic solvents. These conditions may or may not be representative of the interior of a cell where there is a high concentration of biomolecules and DNA is in a highly condensed form. This work focuses on determining the role of DNA condensation on the mechanisms and products of DNA oxidation. DNA is condensed in reverse micelle (RM) media or in the presence of histone subunits. The DNA oxidation reactions are carried out using a flash/quench photochemical technique and ruthenium(II) polypyridyl complexes to produce ruthenium(III) oxidants or singlet oxygen.(43) Maniatis, T.; Fritsch, E. F.; Sambrook, J. Molecular Cloning: A Laboratory Manual; 2 ed.; Cold Spring Harbor Press: Plainview, NY, ... (46) Plum, G. E. In Current Protocols in Nucleic Acid Chemistry; Wiley: New York, 2000, p 7.3.1-7.3. 17.

Title:Ruthenium-mediated DNA Oxidation in Condensed Media
Author:Sarah Elizabeth Evans
Publisher:ProQuest - 2008


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