This hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handling nucleic acids. In easy-to-follow, step-by-step protocols you will learn * how RNAi works in worms, flies and mammals, * how to design the most efficient RNAi constructs, * how to achieve transient, stable and conditional RNAi in cell cultures, * how to determine the efficiency of an RNAi experiment, * and how to use RNAi for gene therapy. All the protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiments and troubleshooting hints to help in establishing your own successful RNAi experiments. Also includes a list of suppliers for RNAi reagents and equipment as well as a glossary of terms.It helps to pre-clone the ORF into the pCR TOPO XL vector using the TOPO XL cloning system for the cloning of extra-large ... manual supplied with the Baculovirus expression system from Gibco-Life Technologies (now Invitrogen). h Note: Foranbsp;...
|Title||:||RNA Interference in Practice|
|Publisher||:||John Wiley & Sons - 2006-03-06|