This methods manual provides an introduction to RNA interference, the theory behind its many applications, and specific protocols for RNAi, in organisms from plants and C.elegans to Drosophila and mammals. There are also chapters covering small hairpin RNAs and viral-induced gene silencing.Protocol. 4.6. RNAi. by. hairpin. transgenes. Hairpin RNAi is an approach that can be considered when it is desirable to target ... pENTR clones can be generated following the protocol outlined in the Gateway Technology Manual ( Invitrogen).
|Author||:||Dr. Tim Doran, Chris Helliwell|
|Publisher||:||CABI - 2009|