The critically acclaimed laboratory standard for more than 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today, truly an essential publication for researchers in all fields of life sciences. This volume and its companions (Volumes 255, 256, 257, and the forthcoming 325 and 329) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.The standard assay that is used to measure the protein kinase activity of JNK and p38 in cells is the immune-complex protein kinase ... To measure endogenous p38 protein kinase activity, we use a rabbit polyclonal antibody raised against the full-length p38 isoform.10 ... that are raised against particular isoforms are not completely specific for those isoforms and show some cross-reactivity with additional isoforms. ... 11 E. Harlow and D. Lane, a#39;a#39;Using Antibodies: A Laboratory Manual.
|Title||:||Regulators and Effectors of Small GTPases, Part F: Ras Family I|
|Publisher||:||Academic Press - 2001-04-17|