The G3 domain of the rat laminin-5 alpha3-chain was expressed in a prokaryotic system engineered with chaperone proteins that allow for soluble protein expression in a cold temperature (11.5AdC) environment. The expressed recombinant G3 (rG3) has a predicted molecular weight (MW) of 27 kDa, which matches the approximated MW for the sequence including the histidine tag. Following expression and verification through Western Blotting, the protein was purified using chromatography.The G3 cDNA was cloned into the pET100 TOPO cloning vector provided with the ChampionTM pET Directional TOPOAr Expression kit (Invitrogen Life Technologies, Carlsbad, CA). The pET100 vector permitted high-level ... Applied Biosystems, Foster City, CA; Turner, 2005). Instructions provided by the ArcticExpressTM Competent Cell manual (Stratagene, La Jolla, CA) were followed for expressionanbsp;...
|Title||:||Recombinant G3 Domain Protein of the Rat Laminin-5 Alpha3 Chain Binds to Integrins on Tumorogenic Breast Cancer Cells to Induce Apoptosis|
|Author||:||Brittany Paige Turner|
|Publisher||:||ProQuest - 2008|