Plant-associated microbes are ubiquitous organisms living in a range of interactions with their host. Involving two organisms, research and applications of plant microbes are challenging and often require specific skills. This book guides the reader in the word of plant-associated fungi, giving both theoretical and practical insight on the potential of this interaction in biotechnology. Detailed instructions and step-by-step protocols are described for isolation, identification, localization and community analysis of fungi, studies on their bioactivity, molecular plant-fungal interactions, and development of fungi as tools for biotechnology.Screen for the positive transformants with the primer pair NS31/AM1 and the following PCR conditions optimised for clone targets: ... with the enzymes HinfI and Hin1II (Fermentas) according to the manufacturera#39;s instructions, and analyse by 3% agarose gel electrophoresis. ... 20 ng), Stoffel buffer (10 mM KCl, 10 mM Tris-HCl, pH 8.3), 0.1 mM dNTPs (Roche Diagnostics GmbH, Mannheim, Germany), 3.75anbsp;...
|Title||:||Prospects and Applications for Plant-Associated Microbes, A laboratory manual|
|Author||:||Anna Maria Pirttilä, Seppo Sorvari|
|Publisher||:||Seppo Sorvari - 2014-12-15|