Probing Lipid Membrane Electrostatics

Probing Lipid Membrane Electrostatics

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The electrostatic properties of lipid bilayer membranes play a significant role in many biological processes. Atomic force microscopy (AFM) is highly sensitive to membrane surface potential in electrolyte solutions. With fully characterized probe tips, AFM can perform quantitative electrostatic analysis of lipid membranes. Electrostatic interactions between Silicon nitride probes and supported zwitterionic dioleoylphosphatidylcholine (DOPC) bilayer with a variable fraction of anionic dioleoylphosphatidylserine (DOPS) were measured by AFM. Classical Gouy-Chapman theory was used to model the membrane electrostatics. The nonlinear Poisson-Boltzmann equation was numerically solved with finite element method to provide the potential distribution around the AFM tips. Theoretical tip-sample electrostatic interactions were calculated with the surface integral of both Maxwell and osmotic stress tensors on tip surface. The measured forces were interpreted with theoretical forces and the resulting surface charge densities of the membrane surfaces were in quantitative agreement with the Gouy-Chapman-Stern model of membrane charge regulation. It was demonstrated that the AFM can quantitatively detect membrane surface potential at a separation of several screening lengths, and that the AFM probe only perturbs the membrane surface potential byFigure 3-5 32 Schematic diagram of tapping mode AFM. Figure 3-6 33 Schematic diagram of force mode AFM. Figure 3-7 34 Schematic diagram of fluid chamber ( not to scale). Figure 3-8 36 Schematic diagram of support lipid bilayer.

Title:Probing Lipid Membrane Electrostatics
Author:Yi Yang
Publisher:ProQuest - 2009


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