Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria.A Laboratory Manual Carlos F. Barbas ... Mix well and store at -80AdC. Centrifuge the remaining volumes of overnight cultures (3800g for 10 minutes at 4AdC), discard the supernatants, and store the pellets at -20AdC for the future preparation ofanbsp;...
|Author||:||Carlos F. Barbas|