This second edition of a much-praised and widely used manual has been entirely revised and updated. Each technique is presented with extensive background information, advice, and troubleshooting. All current applications of PCR are covered, in protocols that have the hallmark reliability of the previous edition.Increase the pre-PCR denaturation step at 94AdC to 10 minutes to inactivate UDG and to hydrolyze the contaminant DNA (Hohlfeld et al. 1994 ... All of Rochea#39;s PCR diagnostic tests utilize a decontamination protocol based on UDG application.
|Author||:||Carl W. Dieffenbach, Gabriela S. Dveksler|
|Publisher||:||Cold Spring Harbor Laboratory Pr - 2003|