Hands-on laboratory experts present a set of qclassicq PCR-based methods for the identification and detection of important animal and food microbial pathogens, including several zoonotic agents. These proven techniques can be precisely applied to a wide variety of microbes, among them Campylobacter spp., chlamydiae, toxigenic clostridia, Escherichia coli (STEC), Listeria monocytogenes, mycoplasmas, salmonellae, and Yersinia enterocolitica. Additional chapters review the specificity and performance of diagnostic PCR analysis, the pre-PCR processing of samples, the critical aspects of standardizing PCR methods, and the general issues involved in using PCR technology for microbial diagnosis.This book provides microbiologists and biochemists with state-of-the-art protocols for the use of PCR-based identification and detection of important bacterial and other microbial pathogens.
|Title||:||PCR Detection of Microbial Pathogens|
|Author||:||Konrad Sachse, Joachim Frey|
|Publisher||:||Springer Science & Business Media - 2003|