Molecular Microbial Ecology Manual

Molecular Microbial Ecology Manual

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After an initial denaturation step (94AdC, 5 min), each PCR cycle consists of denaturation at 94AdC (30 s), annealing at 55AdC ... (10 min in boiling water, then chill on ice) and transfer to a nylon membrane (e.g. of Roche Molecular Biochemicals).

Title:Molecular Microbial Ecology Manual
Author:Antoon D. L. Akkermans, Jan D. Van Elsas, Frans De Bruijn
Publisher:Springer Science & Business Media - 2001-01-01


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