The sequencing of Treponema pallidum subsp. pallidum, the bacterium that causes syphilis, and the identification of a family of 12 genes with sequence similarity that allows scientists to distinguish between treponemal subtypes has opened up a new line of inquiry for biological anthropologists. This research contributes genetic evidence of pre-contact treponematosis in the Americas; by combining osteological and molecular evidence with data on environment and cultural practices, it also furthers our knowledge of human-pathogen interaction.Cloning: Cloning allows for the isolation of individual PCR-amplified molecules, in order to separate multiple products formed during the PGR, so that each can be screened independently. All cloning used the TOPO TA CloningAr Kit for Sequencing (Invitrogen Corporation, Carisbad, CA.; cat. no. K4575). ... The reagents, their measurements, and components are in the manufacturera#39;s instruction manual.
|Title||:||Molecular Identification and Analysis of Treponematosis (syphilis, Bejel, Yaws, Or Pinta) in Ancient Mummified Remains from Northern Chile and Southern Peru|
|Publisher||:||ProQuest - 2008|