Advances in genomic and proteomic profiling of disease have transformed the field of molecular diagnostics, thus leading the way for a major revolution in clinical practice. While the range of tests for disease detection and staging is rapidly expanding, many physicians lack the knowledge required to determine which tests to order and how to interpret results. This handbook provides a complete guide to the use and interpretation of molecular testing in the clinical arena. No other available resource offers this emphasis, comprehensive scope, and practical utility in the clinical setting. * Serves as the definitivereference for molecular pathologists worldwide * Covers a variety of molecular techniques including next generation sequencing, tumor somatic cell genotyping, infectious and genetic disease tecting, and pharmacogenetics * Discusses in the detail issues concerning quality assurance, regulation, ethics, and future directions for the science... is extracted manually. RT-PCR is performed using one enzyme, Thermus thermophilus DNA polymerase (rTth pol), for both RT and DNA polymerase activities. ... Detection uses the same method as the manual assay version ( Roche, 2001).
|Author||:||Wayne W. Grody, Robert M. Nakamura, Frederick L. Kiechle, Charles Strom|
|Publisher||:||Academic Press - 2009-11-06|