Unlike other handbooks in this emerging field, this guide focuses on the challenges and critical parameters in running a metabolomics study, including such often-neglected issues as sample preparation, choice of separation and detection method, recording and evaluating data as well as method validation. By systematically covering the entire workflow, from sample preparation to data processing, the insight and advice offered here helps to clear the hurdles in setting up and running a successful analysis, resulting in high-quality data from every experiment. Based on more than a decade of practical experience in developing, optimizing and validating metabolomics approaches as a routine technology in the academic and industrial research laboratory, the lessons taught here are highly relevant for all systems-level approaches, whether in systems biology, biotechnology, toxicology or pharmaceutical sciences. From the Contents: * Sampling and Sample Preparation in Microbial Metabolomics * Tandem Mass Spectrometry Hyphenated with HPLC and UHPLC for Targeted Metabolomics * GC-MS, LC-MS, CE-MS and Ultrahigh Resolution MS (FTICR-MS) in Metabolomics * NMR-based metabolomics analysis * Potential of Microfluidics and Single Cell Analysis in Metabolomics * Data Processing in Metabolomics * Validation and Measurement Uncertainty in Metabolomic Studies * Metabolomics and its Role in the Study of Mammalian Systems and in Plant Sciences * Metabolomics in Biotechnology and Nutritional Metabolomics and more.Our FAME GC-MS method, performed on an Agilent 6890 GC and 5975 MSD, uses a highly polar cyanopropyl silicone column (HP-88 100 ml X 0.25 mmID X 0.2 pm df, Agilent Technologies) ... The identification strategy consisted of the analysis of FAME reference mixtures complemented with spectral searches in the NIST05 and WILEY275 libraries and manual interpretation of El and positive CI spectra.
|Title||:||Metabolomics in Practice|
|Author||:||Michael Lämmerhofer, Wolfram Weckwerth|
|Publisher||:||John Wiley & Sons - 2013-02-14|