The second study cultured PHH between two layers of PLECM and compared with Matrigel double gel cultures and adsorbed type-1 collagen. Albumin secretion, hepatic transport activity and ammonia metabolism were used as markers of hepatocyte functionality. PHH cultured between two layers of porcine liver ECM had significantly higher levels of albumin secretion, hepatic transport activity, and ammonia metabolism compared to PHH cultured on collagen.UNIVERSITY OF PITTSBURGH SWANSON SCHOOL OF ENGINEERING This dissertation was presented by Tiffany L. Sellaro It was defended on April 30, 2008 and approved by Donna Beer-Stolz, PhD, Department of Cell Biology andanbsp;...
|Title||:||Maintenance of Primary Human Hepatocyte Function in Vitro by Extracellular Matrix Scaffolds|
|Author||:||Tiffany L. Sellaro|
|Publisher||:||ProQuest - 2008|