Subsequently, trypsin was added into the denatured protein sample to digest the extracted Darbe and/or rhEPO into peptide fragments generating T9 peptide from Darbe which was specific and unique to Darbe and the T5 peptide from rhEPO which was specific and unique to rhEPO. The trypsin digestion also generated T17 and T6 peptides from rhEPO and Darbe which were specific and unique to both rhEPO and Darbe. This work found that doubling the trypsin concentration in trypsin digestion method from 2 mug (24.7 mug per mL of digest) to 4 mug (49.4 mug per mL of digest) increased the detection of T17, T6, and T9 peptides from 1177, 821, 7385 integrated peak mass areas for T17, T6, and T9 peptides, respectively, to 2653, 857, and 7518 integrated peak mass areas for T17, T6, and T9 peptides, respectively.(2007 aamp; 2008) references and according to the manufacturera#39;s manual from Invitrogen. ... Legen Mach 1.6 R centrifuge with fixed angel rotors (Thermo- Kendro Laboratory Products Inc., Asheville, NC), and Isotemp incubator (Fisher Scientific, anbsp;...
|Title||:||LC-MS Techniques for Analytical Testing of Recombinant Human Erythropoietin and Darbepoetin Alfa in Equine Plasma Samples for Anti-doping Control|
|Publisher||:||ProQuest - 2008|