Hormone Assays in Biological Fluids

Hormone Assays in Biological Fluids

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Because of the development of commercial hormone assays the need for a pure methodology book has been overlooked. There is a need for advice about commerical assays as well as protocols for manual assays that can offer greater specificity or sensitivity. Hormone Assays in Biological Fluids fills this need and provides a range of methods for the different hormones, and also highlights the pitfalls of current measurement approaches and solutions to the difficulties of hormone measurement. It will save analysts time in deciding which is the best method to use and will help avoid many of the most common and time wasting mistakes. Commercial assays both manual and automated, are available for the majority of hormones, still there is confusion over the reliability and specificity of these methods. This text presents a guide to both commercial assays and manual assays thereby assuring specificity and accuracy of measurement. Analysts will find in this book clear guidance on the best method to use for their particular study. Hormone Assays in Biological Fluids will also prove to be an invaluable tool for pharmacologists involved in clinical trials, students, and practicing physicians: It will help researchers choose the best method for their particular research purposes, and be applicable to a wide range of scientists, including biochemists, immunologists, physiologists, pharmacologists and research clinicians who are involved in endocrine research.Figure 1 shows a chromatographic profile of a sample that gave a result of 1800 mIU/L (reference range for normals alt; 500 mIU/L). In the Wallac DELFIA assay, the prolactin was only 700 mIU/L. After PEG precipitation the Bayer Advia Centauranbsp;...

Title:Hormone Assays in Biological Fluids
Author:M. J. Wheeler
Publisher:Springer Science & Business Media - 2006


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