This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.Protocol 3 provides condensed guidelines for transiently transfecting cells. Molecular cloning books and lab manuals, such as Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press), describe procedures for makinganbsp;...
|Title||:||Handbook of Single-Molecule Biophysics|
|Author||:||Peter Hinterdorfer, Antoine van Oijen|
|Publisher||:||Springer Science & Business Media - 2009-12-24|