Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study qYour Favorite Geneq in yeast. Key Features * Basic Methods in Yeast Genetics * Physical and genetic mapping * Making and recovering mutants * Cloning and Recombinant DNA Methods * High-efficiency transformation * Preparation of yeast artificial chromosome vectors * Basic Methods of Cell Biology * Immunomicroscopy * Protein targeting assays * Biochemistry of Gene Expression * Vectors for regulated expression * Isolation of labeled and unlabeled DNA, RNA, and proteinEnsure that the safety cover is in place during operation and that hands and loose clothing are kept well free of the moving parts. 4. Grow the query plate ... Load the hopper with the appropriate number of 384 short pin pads. Start the ... The query and/or array source dishes can continue to be used as needed. Critical step.
|Title||:||Guide to Yeast Genetics and Molecular Biology|
|Publisher||:||Gulf Professional Publishing - 2004-05-14|