the cell wall, coating them with concanavalin A or cationic silica microbeads to stabilize the plasma membrane, followed by lysis. We have ... (Because some pmal mutants are sensitive to the pH of the growth medium, it is important to be aware of pH differences between synthetic medium and YPD.) ... Just before use, add 1 mM phenyl- methylsulfonyl fluoride (PMSF) (from a 250 mM stock in ethanol).
|Title||:||Guide to Yeast Genetics and Molecular and Cell Biology|
|Author||:||Christine Guthrie, Gerald R. Fink|
|Publisher||:||Gulf Professional Publishing - 2002|