In the present work, we report a novel 10 liter scale purification scheme resulting in highly purified, stable, concentrated solutions of engineered protein rods. This process is scalable and well suited for production use. In addition, we describe the ability to control the length of the tail fiber after its production using bacteriophage infection via site-specific protease cleavage. Also described is the in vivo biotinylation of the fiber rods at discrete locations, providing a versatile attachment strategy with highly accurate nanoscale spatial control.Sambrook, J.; Fritsch, E. F.; Maniatis, T., Molecular cloning: a laboratory manual. 2nd ed.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, N.Y., 1989. 19. Stafford, W. F. ... Molecular Biology 1987, 21, (5), 1040- 1048. 23. Ward, S.; Luftig ... Journal Of Biological Chemistry 1964, 239, (1), 269-273. 27. Edgar, R. S. anbsp;...
|Title||:||Engineered Bacteriophage T4 Tail Fiber Proteins for Nanotechnology|
|Publisher||:||ProQuest - 2008|