Carbon dioxide was introduced into the fermentation chamber of the chemi-visual sensor while the pH and red-green-blue (RGB) color values of the phenol red indicator solution were recorded. A CCD camera (WebCam) and image analysis software package developed in MatlabRTM was used to record the RGB values of the chemi-visual solution at each CO2 loading. Calibration curves were developed for the following relationships: CO2 vs. pH, pH vs. RGB, and CO2 vs. RGB.... the effective diameter of the membrane in contact with indicator solution and gaseous headspace was 15 mm. An Accumet Basic AB15/15+ pH meter was used to record the pH of the indicator solution at each carbon dioxide concentration.
|Title||:||Development of a High-throughput Fermentation Assay Using Colorimetric Measurement of Gas Production|
|Author||:||Steven Thomas Bly|
|Publisher||:||ProQuest - 2008|