This new volume of Methods in Cell Biology looks at methods for analyzing correlative light and electron microscopy (CLEM). With CLEM, people try to combine the advantages of both worlds, i.e. the dynamics information obtained by light microscopy and the ultrastructure as provided by electron microscopy. This volume contains the latest techniques on correlative microscopy showing that combining two imaging modalities provides more than each technique alone. Most importantly it includes the essential protocols, including tips, tricks and images for you to repeat these exciting techniques in your own lab. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material Second of two volumes dedicated to Correlative Light and Electron microscopy (CLEM)Individual actin filaments were manually segmented across the tomogram using the embedded drawing tools. Z-scale of the ... MATERIALS. Instrumentation: Emitech K950C carbon coater; Heating oven (Binder WTC, Tubbingen, Germany) .
|Title||:||Correlative Light and Electron Microscopy II|
|Publisher||:||Academic Press - 2014-09-30|