Proceedings of the 7th International Conference on Coelenterate Biolology, held at the University of Kansas, Lawrence, Kansas, USA, 6-11 July 2003The purpose of this study is to report the cloning and sequence analysis of GS cDNA from unfed A. pallida. ... two-four animals (100 mg) for 5 min in a glass manual tissue grinder on ice in 5 volumes (0.5 ml) of buffer containing 10 mM Tris (pH 7.0) and 560 mM NaCl. ... The resulting RT-PCR product (1000 bp) was cloned into pCRII-TOPO (Invitrogen, Inc.), and the complete sequence analysisanbsp;...
|Title||:||Coelenterate Biology 2003|
|Author||:||Daphne G. Fautin, Jane A. Westfall, Paulyn Cartwright, Marymegan Daly, Charles R. Wyttenbach|
|Publisher||:||Springer Science & Business Media - 2005-03-07|