Scope and method of study. This study characterized heme lipoprotein (HeLp), the most abundant protein in Ixodid tick hemolymph and saliva using a number of biochemical and molecular methods.A QIAquickAr gel extraction kit (Qiagen) was used to isolate DNA from gel pieces, following the provided protocol. ... In one instance, a TOPO TA CloningAr kit ( Invitrogen) was used to clone a PCR product following the included instructions.
|Title||:||Characterization of Heme Lipoprotein in Ixodid Tick Saliva and Hemolymph|
|Author||:||William Justin Cordill|
|Publisher||:||ProQuest - 2007|