Cell-free protein synthesis is coming of age! Motivated by an escalating need for efficient protein synthesis and empowered by readily accessible cell-free protein synthesis kits, the technology is expanding both in the range of feasible proteins and in the ways that proteins can be labeled and modified. This volume follows qCell-Free Translation Systemsq, edited by Professor Alexander S. Spirin in 2002. Since then, an impressive collection of new work has emerged that demonstrates a substantial expansion of capability. In this volume, we show that proteins now can be efficiently produced using PCR products as DNA templates and that even membrane proteins and proteins with multiple disulfide proteins are obtained at high yields. Many additional advances are also presented. It is an exciting time for protein synthesis technology.Here, the effect of several PCR additives described to enhance specificity of amplification was investigated with respect to primer-dimer formation. Materials and ... (Roche Applied Science) was used as recommended in the instruction manual.
|Title||:||Cell-Free Protein Expression|
|Author||:||James R. Swartz|
|Publisher||:||Springer Science & Business Media - 2012-12-06|