This edition emphasizes the decisions that need to be made to select one procedure over another in the everyday workings of a molecular biology laboratory. Trouble-shooting problems that may emerge when performing such methods are included and concise instructions are provided.Melt LB top agar using an autoclave or microwave oven. Three mL of top agar will be needed for each ... Refer to Commentary in Section 15-3 for a guide to troubleshooting problems with the ligation. Section 15=5 Screening Ml 3 Subclonesanbsp;...
|Title||:||Basic Methods in Molecular Biology|
|Author||:||Leonard G. Davis, W. Michael Kuehl, James F. Battey|