Glycerol 3-phosphate dehydrogenase (G3PDH) and sarco/endoplasmic reticulum Ca2+ channel ATPase (SERCA) were chosen for phylogenetic analysis. These genes have low copy numbers, ubiquitous expression, and similar substitution rates among taxa, which complement the substitution rate of SSU. These genes were sequenced from 17 metazoan phyla for comparison to SSU phylogenies. Various combinations of the new datasets and a dataset of SSU sequences from comparable taxa were examined using Bayesian, Maximum Likelihood and Neighbor Joining phylogenetic methods.A TOPO TA Cloning Kit for Sequencing (Invitrogen, Carlsbad, CA) contains: pCR Ar 4-TOPO vector, salt solution, SOC broth and TOP10 competent E. coli cells. The instruction manual calls for reactions twice the size of the one described here ;anbsp;...
|Title||:||Analysis of Glycerol 3-phosphate Dehydrogenase and Sarco/endoplasmic Reticulum Calcium Ion Channel ATPase for Use in Deep Metazoan Phylogenetics|
|Author||:||Terry G. Campbell|
|Publisher||:||ProQuest - 2008|