This book comprehensively describes the development and practice of DNA-encoded library synthesis technology. Together, the chapters detail an approach to drug discovery that offers an attractive addition to the portfolio of existing hit generation technologies such as high-throughput screening, structure-based drug discovery and fragment-based screening. The book: Provides a valuable guide for understanding and applying DNA-encoded combinatorial chemistry Helps chemists generate and screen novel chemical libraries of large size and quality Bridges interdisciplinary areas of DNA-encoded combinatorial chemistry a synthetic and analytical chemistry, molecular biology, informatics, and biochemistry Shows medicinal and pharmaceutical chemists how to efficiently broaden available achemical spacea for drug discovery Provides expert and up-to-date summary of reported literature for DNA-encoded and DNA-directed chemistry technology and methods... temperature 30.4 Analyze by LC/MS Step Pool 31 Pool all 96 wells into a single vessel Step Ethanol crash 32 Add Volume Total volume 32.1 5 M NaCl(aq) 270 I¼L 2.97 mL 32.2 Cold EtOH 5.93 mL 8.90 mL 32.3 a78AdC for ay30 min or a20 AdCanbsp;...
|Title||:||A Handbook for DNA-Encoded Chemistry|
|Author||:||Robert A. Goodnow, Jr.|
|Publisher||:||John Wiley & Sons - 2014-04-10|