At last . a collection of practical protocols for explanting and manipulating neuronal and glial cells. A Dissection and Tissue Culture Manual of the Nervous System Abraham Shahar, Jean de Vellis, Antonia Vernadakis, and Bernard Haber, Editors Among research laboratories involved with neuronal and glial cell cultures and their applications, there is a growing demand for a hand-book describing dissection procedures, culture preparation techniques, and the in vitro manipulation of neural cells and tissues for specific analytical purposes. A Dissection and Tissue Culture Manual of the Nervous System offers a diverse collection of methods that have been developed by and are used routinely within specialized neurobiological laboratories. Written in an easy-to-follow style, the procedures described in this unique guide are designed by experts to be applied by those with limited experience in the field. Organized into ten comprehensive sections, ninety concise contributions from leading laboratories worldwide put forth practical, stepwise protocols for neural cell manipulation and experimentation. Methods encompass: * an illustrated outline of techniques for the dissection of brain areas in the fetus and the neonate * the dissection of selected specialized structures, such as the ciliary ganglion * organotypic. explant culture of nervous tissue * dissociated culture of astrocytes, oligodendrocyte, neurons, and Schwann cells * reaggregation culture of dissociated cells. Sections devoted to various tissue processing methods and experimental applications of cultured material present histochemical, autoradio-graphic, and immunocytochemical staining and visualization techniques. In situ hybridization methods, as well as preparative procedures for electron microscopy and biochemical and physiological assays, are discussed with an emphasis on methods tailored for the neurobiologist. Alternative techniques for the cultivation of the same organ or cell type from diverse animal species are juxtaposed with a varied selection of methodology and instrumentation, and complemented by key literature citations for further reading, to enable the investigator to chose the appropriate approach for a specific neurobiological application. Presented in a comb-bound format for convenient use on the laboratory bench, A Dissection and Tissue Culture Manual of the Nearvous System will be an essential research companion to graduate students, post-doctoral fellows and other laborabory investigators in cell and developmental neurobiology, neuroanatomy, neurophysiology, neuropharmacology, and biochemistry.Preincubate for 25a30 min in a diaminobenzidine solution [Graham and Karnovsky, 1966]. Dissolve 15 mg ... To enhance the staining, add 0.75 ml 1% cobalt chloride and then 0.6 ml 1% nickel ammonium sulfate to this solution [ Adams, 1981].
|Title||:||A Dissection and Tissue Culture Manual of the Nervous System|
|Publisher||:||Wiley-Liss - 1989-12-05|